Solvent system: PEG (MW=1000) 12.5%/K2HPO4 12.5%, two-aqueous phases, PEG in upper layer, CCC run with lower phase mobile, upper phase stationary,1 ml/min, 840 rpm detection at 280 nm.

CCC sample load: Myoglobin (17.67 kDa) 25 mg and

Lysozyme (14.39 kDa) 31 mg,

       

 

1. Gibco low MW std

2. Fraction 20 – solvent font ~ 30 min

3. Fraction 41— 82 min

4. 48 – 96 min

5. 53 – 106 min

6. 69 – 137 min

7. 88 – 176 min

8.1 00 –200 min

9. 112 – 224 min

10. Lysozyme, 2 ug

11. Myoglobin, 2ug 

PAGE samples in aqueous phase in high conc. K2HPO4

5 ul of each sample run non-reduced on a 10-20% Tricine gel