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Spiral CCC separation of Small Molecules
Mix of 18-44 mg 3 compounds.
Solvent system: Hexane-ethyl acetate-methanol-water (3:5:3:5 by vol)
Elution mode: U-o-T, CW
840 rpm, flow = 1.3 ml/min, 4 min fractions, manual OD readings. SF= 70.4 Identification by HPLC (below).
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