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Spiral CCC separation of Small Molecules




Mix of 18-44 mg 3 compounds.

Solvent system: Hexane-ethyl acetate-methanol-water (3:5:3:5 by vol)

Elution mode: U-o-T, CW

840 rpm, flow = 1.3 ml/min, 4 min fractions, manual OD readings. SF= 70.4 Identification by HPLC (below).




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