Spiral CCC separation of Small Molecules

Mix of 18-44 mg 3 compounds. Solvent system: Hexane-ethyl acetate-methanol-water (3:5:3:5 by vol) Elution mode: U-o-T, CW 840 rpm, flow = 1.3 ml/min, 4 min fractions, manual OD readings. SF= 70.4 Identification by HPLC (below).